Scar
Stop

Part:BBa_K1362473:Design

Designed by: Jan Gleixner   Group: iGEM14_Heidelberg   (2014-10-09)


C terminal Stop insert for RFC[???] N intein constructs with two insertion sites


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As a sequence of length 0 could not be cloned as an oligo into an RFC[???] the sequence starts with two stop codons to end the future POI-C intein cds as soon as possible. For better oligo annealing and thus ligation the sequence was extended for 6 nucleotides.


Source

References